Disclaimer. OR. Flint, J. 2014 Jun;68(6):743-50. doi: 10.1007/s00284-014-0531-x. This is consistent with the promoter of the full-length gene being present upstream from the EFCAB13 version, which is supported by H3K4me3 analysis (Supplementary Fig. Gene copy-number polymorphism caused by retrotransposition in humans. AugustusPB transcripts that could not be confidently assigned to parent transcripts were discarded and not evaluated for novel contribution. The assemblies also showed extensive diversity at each of the other loci examined: immunity-related GTPases (IRGs) and Schlafen family (Slfn). 2009 Jan;31(1):65-9. doi: 10.1007/s10529-008-9828-9. We also created a de novo set of strain-specific genes and isoforms from Comparative Augustus (AugustusCGP)24 using the strain-specific RNA-Seq and the progressive Cactus alignment. Scaffolds were broken in locations where there was not a minimum number of 10 kb and 40 kb (where available) fragments that spanned the join. Cas9 mRNA (Trilink) together with the four gRNAs was injected into the cytoplasm of single-cell C57BL/6NTac zygotes. Only coordinates in which at least one strain had a hSNP call were retained. If multiple assignments were created, attempts to resolve them were made by finding if any gene had a Jaccard distance 0.2 greater than any other; otherwise, they were discarded. 14). Terpenes are aromatic compounds found in cannabis and other plants that contribute to each strain's unique scent and flavor profile. Gene retrotransposition has long been implicated in the creation of gene family diversity36, novel alleles conferring positively selected adaptations37. Behav. The remaining 31 strains used are commercial starters obtained from different companies. USA 102, 814 (2005). Seq. Some suggest Red Velvet is a mix of Lemon Cherry Gelato and Pina Acai or a cross between Orange Velvet and Redrum. The material provided on Leafly is intended for educational and informational purposes only. Genet. Proc. a, Summary of the strain-specific gene sets showing the number of genes broken down by GENCODE biotype. Your reply will be published soon after verification. Epub 2003 Jun 24. But with the Headspace weed strain, you'll also get a side effect that is like your friend's annoying habit of always showing up late drowsiness. Approximately 90% of intergenic regions in the PWK/PhJ assembly of the Nlrp1 locus is composed of transposable elements (Fig. c, Novel protein-coding alleles of the Nlrp1 gene family in the wild-derived strains and two classical inbred strains. 2019 Apr;249(4):1007-1015. doi: 10.1007/s00425-018-3057-9. Metabolism of hexahydro-1,3,5-trinitro-1,3,5-triazine through initial reduction to hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine followed by denitration in Clostridium bifermentans HAW-1. Clamp the barrel, padded, upright in your bench vise. The scaffolds were assembled into chromosome-scale scaffolds using Ragout v2.0. Headspace has a modest amount of 19% THC on average although this number could vary from one grower to another. The most well-known cannabinoids are THC (tetrahydrocannabinol), which is responsible for the psychoactive effects of cannabis, and CBD (cannabidiol), which is non-intoxicating and known for its potential therapeutic properties. For non-coding transcripts, the fewest number of transcripts to keep at least 80% of present non-coding splice junctions were retained. 4) that appear to have arisen either through retrotransposition (~80%) or gene duplication events (~20%). Initial sequencing and comparative analysis of the mouse genome. These comparative transcripts were given to the gene-finding tool AUGUSTUS13 as strong hints (external evidence) in conjunction with weaker hints derived from all available RNA-Seq data for the given strain. Sequence accessions for the three immune-related loci on chromosome 11 are available from the European Nucleotide Archive (Supplementary Table 23). Semin. J. Immunol. Giordano, J. et al. You might feel like you want to take a nap after smoking this weed, but don't worry the happiness will keep you from actually falling asleep. 5) and accounted for ~49% of all hSNPs (Supplementary Table 9 and Supplementary Fig. 6a). The total terpene content is impressive, at 1.00% of the total weight. WebHeadspace is a new hybrid marijuana strain that has become popular for its libido-boosting effects. Immunol. 5). We thank V. Vancollie for assistance with phenotyping data. Interestingly, within this region, Cpsf3 (21.29 Mb) is located on an island of high conservation in all strains and a homozygous C57BL/6NJ knockout produces subviable offspring35. We'll explore top-quality strains, knowledgeable budtenders, and more. Physiol. First, these loci were compared to the respective regions in the C57BL/6NJ assembly and used to identify contigs from public assemblies of the reference strain previously omitted due to insufficient overlap. Across all strains, hSNP regions account for 1.55.5% of protein-coding genes (Fig. Schrider, D. R. et al. Biol. We have completed the first draft de novo assemblies and strain-specific gene annotation for 12 classical inbred laboratory mouse strains (129S1/SvImJ, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NZO/HlLtJ, and NOD/ShiLtJ) and 4 wild-derived strains representing the backgrounds Mus musculus castaneus (CAST/EiJ), M. m. musculus (PWK/PhJ), M. m. domesticus (WSB/EiJ), and M. spretus (SPRET/EiJ). Transcriptional analysis of the three Nlrp1 paralogs in mice. Churchill, G. A., Gatti, D. M., Munger, S. C. & Svenson, K. L. The Diversity Outbred mouse population. Many hSNP clusters overlap immunity (for example, MHC, NOD-like receptors, and AIM-like receptors), sensory (for example, olfactory and taste receptors), reproductive (for example, pregnancy-specific glycoproteins and sperm-associated E-rich proteins), and neuronal- and behavior-related genes (for example, itch receptors26 and -protocadherins27) (Fig. Google Scholar. Corresponding brain regions are labelled with a number that is described below the panel (Supplementary Table 15). Headspace is a mindfulness app with an easy-to-use interface that takes the seriousness and intimidation out of meditation. The paternal gene of the DDK syndrome maps to the Schlafen gene cluster on mouse chromosome 11. PubMed Central Levinsohn, J. L. et al. Another member present in the reference, Slfn14, is conserved in PWK/PhJ and CAST/EiJ but is a pseudogene in WSB/EiJ (Supplementary Fig. T.M.K., D.J.A., B.P., and J.F. We're still gathering information about the Headspace strain. These transMapped transcript alignments were evaluated by a series of binary classifiers that attempt to diagnose differences between the parent and target genome. European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK, Jingtao Lilue,Anthony G. Doran,Ruth Bennett,Paul Flicek,Adam Frankish,Jane Loveland,David Thybert&Thomas M. Keane, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK, Jingtao Lilue,Anthony G. Doran,Monica Abrudan,William Chow,Joanna Collins,Petr Danecek,Dirk-Dominik Dolle,Matt Dunn,Richard Durbin,Paul Flicek,Adam Frankish,Beiyuan Fu,James Gilbert,Jennifer Harrow,Kerstin Howe,Ximena Ibarra-Soria,Chris J. Lelliott,Darren W. Logan,Jane Loveland,Naomi Park,Sarah Pelan,Mike Quail,Lesley Shirley,Charles Steward,Mark Thomas,Glen Threadgold,James Torrance,Kim Wong,Jonathan Wood,Fengtang Yang,David J. Adams&Thomas M. Keane, Center for Biomolecular Science and Engineering, University of California Santa Cruz, Santa Cruz, CA, USA, Ian T. Fiddes,Joel Armstrong,Mark Diekhans,Dent Earl&Benedict Paten, Institut de Gntique et de Biologie Molculaire et Cellulaire, Centre National de la Recherche Scientifique UMR7104, Institut National de la Sant et de la Recherche Mdicale U964, Universit de Strasbourg, Illkirch, France, Centre des Sciences du Got et de lAlimentation, University of Bourgogne Franche-Comt, Dijon, France, The Jackson Laboratory, Bar Harbor, ME, USA, Department of Genetics, University of Cambridge, Cambridge, UK, Brain Research Institute, University of California, Los Angeles, CA, USA, Yale Computational Biology and Bioinformatics, Yale University, New Haven, CT, USA, Mark Gerstein,Paul Muir,Fabio C. P. Navarro&Cristina Sisu, OxFORD Asset Management, OxAM House, Oxford, UK, Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA, USA, Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL, USA, Genetics Institute, University College London, London, UK, Institute of Mathematics and Computer Science, University of Greifswald, Greifswald, Germany, Stefanie Nachtweide,Lars Romoth&Mario Stanke, Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge, UK, German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics, Heidelberg, Germany, Department of Bioscience, Brunel University London, Uxbridge, UK, Departamento de Biologa Celular y Molecular, Facultad de Ciencias Biolgicas, Pontificia Universidad Catlica de Chile, Santiago, Chile, Earlham Institute, Norwich Research Park, Norwich, UK, School of Life Sciences, University of Nottingham, Nottingham, UK, You can also search for this author in Multiple trait measurements in 43 inbred mouse strains capture the phenotypic diversity characteristic of human populations. Whether you're a seasoned smoker or a curious tourist, our guide can help you find the best cannabis dispensaries anywhere you go. Prepritn available at https://www.biorxiv.org/content/early/2018/02/11/088435 (2018). Raet1 and H60 are important ligands for NKG2D, an activating receptor of natural killer cells43. For example, strains high in THC may produce stronger psychoactive effects, while strains with balanced THC-to-CBD ratios may offer milder effects with potential medical benefits. A novel start codon upstream of Slfn4, which causes a 25 amino acid N-terminal extension, was identified in PWK/PhJ and WSB/EiJ. To establish an expected rate for each protein class, the same analysis was carried out using the entire protein-coding CDS annotated gene set from GENCODE M8. OSullivan, T., Dunn, G. P., Lacoursiere, D. Y., Schreiber, R. D. & Bui, J. D. Cancer immunoediting of the NK group 2D ligand H60a. For the first time, it shows the order, orientation, and structure of three highly divergent haplotypes present in WSB/EiJ, PWK/PhJ, and SPRET/EiJ, including novel annotation of rearranged promoters, inserted processed pseudogenes, and a high frequency of LINE repeats (Supplementary Data 6). Google Scholar. PLoS Comput. If you've had edibles, flower, joints, or other cannabis products of this Both CAST/EiJ and PWK/PhJ contain functional copies of Sfln10, which is a predicted pseudogene in C57BL/6J and WSB/EiJ. For every strain into our database you will be able to create an unique map for all known hybrids and crossings! Anthony DiMeo. 26, 342350 (2016). was supported by a research grant from CONICYT/FONDECYT/REGULAR No.1171004 and the European Commission (EUFP7 BLUEPRINT grant HEALTH-F5-2011-282510). Psychiatry 22, 836849 (2017). d, A regional dot plot of the Nlrp1 locus in PWK/PhJ compared to the C57BL/6J GRCm38 reference (color-coded same as panel c). Thereby, the evidence from t on the location of exons, introns and start and stop codons was given a much higher weight in order to produce the original splice form, also in cases where the majority of target RNA-Seq suggests a different major splice form. Cristina Sisu, Paul Muir, Mark Gerstein, Jobran Chebib, Benjamin C. Jackson, Peter D. Keightley, Shweta Ramdas, Ayse Bilge Ozel, Jun Z. Li, Karen H. Y. Wong, Walfred Ma, Pui-Yan Kwok, Andrew P. Morgan, Jonathan J. Hughes, Fernando Pardo-Manuel de Villena, Arang Rhie, Shane A. McCarthy, Erich D. Jarvis, Bjarni V. Halldorsson, Hannes P. Eggertsson, Kari Stefansson, Alexandre Almeida, Stephen Nayfach, Robert D. Finn, Nature Genetics WGE: a CRISPR database for genome engineering. 306, L10L22 (2014). Slider with three articles shown per slide. The Nlrp1 locus (NOD-like receptors, pyrin domain-containing) encodes inflammasome components that sense endogenous microbial products and metabolic stresses, thereby stimulating innate immune responses51. You'll be able to concentrate on whatever it is that's important to you while remaining in an uplifted mood. These divergent profiles suggest that Nlrp1c is not the sole mediator of anthrax lethal toxin resistance in the mouse but several other members may be involved. Leafly is not engaged in rendering medical service or advice and the information provided is not a substitute for a professional medical opinion. Mamm. The wild-derived PWK/PhJ (M. m. musculus) and CAST/EiJ (M. m. castaneus) strains share highly similar haplotypes; however, PWK/PhJ macrophages are resistant to pyroptotic cell death induced by anthrax lethal toxin while CAST/EiJ macrophages are not54. Genome Res. From the pure landraces up to the last hybrid! Hybrid strains are a combination of Indica and Sativa genetics and can offer a balance of effects, with various ratios available (e.g., Indica-dominant, Sativa-dominant, or balanced). Once transposed, transposable elements accumulate mutations over time as the sequence diverges38,39. However, it's important to start with small amounts and monitor your reactions, as individual responses to cannabis can vary. Genetics 185, 10811095 (2010). A cluster of approximately 50 genes, which includes hippocalcin-like 1 (Hpcal1) and its homologs, were identified (chromosome 12: 1825 Mb). J. Physiol. For example, chromosome 10 (22.122.4 Mb) on C57BL/6J contains Raet1 alleles and minor histocompatibility antigen members of H60. 1e). WebAfter cultivation with 21% (v/v) O2 in the headspace, no recombinant hydrogenase activity was observed in soluble extracts of wild-type BL21, although low levels of activity could be Kelada, S. N. P. et al. Members of group 4 Slfn genes50, Slfn8, Slfn9, and Slfn10, show significant sequence diversity among these strains. Fixed chromatin was then digested with restriction enzyme Mbo I, the 5 overhangs were filled in with biotinylated nucleotides and then free blunt-ends were ligated. 1b and Supplementary Fig. Uteroglobin: a potential novel tumor suppressor and molecular therapeutic for prostate cancer. Bruce Banner is a powerful hybrid cross between Strawberry Diesel and Ghosts cut of OG Kush. Nature 420, 520562 (2002). FOIA The expected effects are a little bit like the effects of a good friend: relaxed, happy, euphoric, uplifted, creative, and focused. A digital atlas of gene expression patterns in the mouse: http://www.genepaint.org, A pipeline used to comparatively annotate the mouse strains for the Mouse Genomes Project: https://github.com/ucsc-mus-strain-cactus/MouseGenomesAnnotationPipeline, SGA String Graph Assembler a de novo genome assembler: https://github.com/jts/sga, SNAP Scalable Nucleotide Alignment Program a new sequence aligner: http://snap.cs.berkeley.edu, ImageJ an image processing toolkit: https://imagej.nih.gov/ij/. Comparative gene prediction identified the full-length version in orangutan, rhesus macaque, bushbaby, and squirrel monkey. If material is not included in the articles Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Science 346, 987991 (2014). PLoS Genet. On average, 10% of synteny block adjacencies in the assembled genomes were not presented in C57BL/6J reference. For each transMapped transcript alignment t, one way to identify its structure was a pipeline component we here refer to as AugustusTMR (TM=transMap, R=RNA-Seq). Chromosome-scale shotgun assembly using an in vitro method for long-range linkage. Be mindful of the method of consumption (e.g., smoking, vaping, edibles) as it can impact the onset and duration of effects. Keller, O., Kollmar, M., Stanke, M. & Waack, S. A novel hybrid gene prediction method employing protein multiple sequence alignments. Proc. This makes it possible to exploit the combined evidence for gene finding and to discover genes that, for example, are only weakly expressed and partially supported in the reference strain but that have a high expression in other strains. Medical marijuana You must take responsibility for abiding the law of the city, country or the jurisdiction where you are located. In addition to Aspen bedding substrate, standard environmental enrichment of a nestlet and a cardboard tunnel were provided. https://doi.org/10.1101/231118. Histograms showing the neuroanatomical features as percentage increase or decrease of the assessed brain regions in Efcab3-like mutant mice compared to matched controls (right). Host genetic diversity enables Ebola hemorrhagic fever pathogenesis and resistance. Genome Res. Health Perspect. If no complete transcripts are present, one longest CDS is picked for the gene. Also, if you've been struggling with weight loss and don't know how to get out of the slump that comes with losing motivation to eat healthily, try this strain. Mamm. Discover 7 surprising ways to use hemp in your daily life, from beauty products to food. Liebenauer, L. L. & Slotnick, B. M. Social organization and aggression in a group of olfactory bulbectomized male mice. Simultaneous access to many rodent species assemblies, in parallel with individual-level gene predictions, expression and long-read data, facilitated the accurate prediction of many strain-specific haplotypes and gene isoforms. Medical patients choose Head space to relieve symptoms of stress, pain and anxiety according the growers who claim this produces dark green leaves dotted with trichomes while burnt orange hairs on them as well! By accessing this site, you accept the Terms of Use and Privacy Policy. Protoc. This Efcab3-like gene was manually curated, validated according to HAVANA guidelines61 and identified in GENCODE releases M11 onwards as Gm11639. Most often these appear to result from gene duplication events. Based on these results, samples were selected for odor evaluation by 55 sensory panelists in work carried out by Headspace Sensorys Gilbert. An improved approach to mate-paired library preparation for Illumina sequencing. Clostridium geopurificans strain MJ1 sp. 3, e137 (2007). Interestingly, the lateral ventricle was one the most severely affected brain structures exhibiting an enlargement of 65% (P=0.007). The RNA-Seq hints were generated for each of the novel strains by aligning RNA-Seq reads to the native genome with the spliced aligner STAR16. Cell Tissue Res. Population variation in NAIP functional copy number confers increased cell death upon Legionella pneumophila infection. 21, 15121528 (2011). Genetic diversity at gene loci, particularly those related to defence and immunity, is often the result of selection that, if retained, can lead to the rise of divergent alleles in a population64. In particular, the wild-derived strains represent a rich resource of novel target sites, resistance alleles, genes and isoforms not present in the reference strain, or indeed WebHeadspace is a relatively new hybrid marijuana strain. If you're looking for a strain that will help with your pain without making you feel high, this one is for you! 84, 81728180 (2010). Mus is the first mammalian lineage to have multiple chromosome-scale genomes. 35, 222236 (2015). Putnam, N. H. et al. Additional examples include another region on chromosome 12 (8788 Mb) containing approximately 20 eukaryotic translation initiation factor 1A (eIF1a) homologs and on chromosome 14 (4145 Mb) containing approximately 100 Dlg1-like genes. PLoS Pathog. Ragout classified 38% of them as valid rearrangements and the rest as misassemblies (which were removed). PubMed The representation of known mouse repeat families in the assemblies shows that the short repeat (<200 base pairs (bp)) content was comparable to GRCm38 (Supplementary Fig. M.Q., L.S., N.P., L.Re., A.C., M.Du., and A.F.-S. prepared the samples and carried out the sequencing. A consensus-finding algorithm was employed to decide between possible versions of an orthologous transcript. Hum. We examined retrotransposon content in hSNP dense regions on GRCm38 compared to an estimated null distribution (one million simulations) and found a significant enrichment of both LTRs (empirical P<1107) and long interspersed nuclear elements (LINEs) (empirical P<1107) (Supplementary Tables 11 and 12). 1d, left). Initial sequencing and analysis of the human genome. Newly annotated members Nlrp1b2 and Nlrp1d appear functionally intact in CAST/EiJ but were both predicted as pseudogenes in PWK/PhJ due to the presence of stop codons or frameshift mutations. 1e). Every sequencing lane was genotype checked against the mouse Hapmap SNP calls71 using the Samtools/Bcftools v1.1 'gtcheck' command. The research leading to these results has received funding from the European Unions Seventh Framework Programme (FP7/20072013) under grant agreement HEALTH-F4-2010-241504 (EURATRANS). Second, C57BL/6J reads aligned to the regions of interest in the C57BL/6NJ assembly were extracted for targeted assembly, leading to the generation of contigs covering sequences currently missing from the reference. We examined three immunity-related loci on chromosome 11, IRG (GRCm38: 48.8549.10 Mb), Nlrp1 (71.0571.30 Mb), and Slfn (82.983.3 Mb) because of their polymorphic complexity and importance for mouse survival48,49,50. 1950 187, 35383545 (2011). Skip to Genealogies of mouse inbred strains. The progressiveCactus whole genome alignments were used to project annotations from GENCODE VM860 onto each of the strain-specific assemblies using transMap75. Pairs of flanking guide RNAs (gRNAs) were designed using the WTSI Genome Editing (WGE) tool78 creating four gRNAs (two gRNAs 5 and two gRNAs 3 to the CE region, Supplementary Table 21). Briefly, the constitutive coding exon 5 (chromosome 11: 104700610-104700692, GRCm38), which is well-supported by RNA-Seq data in multiple tissues (ENSMUST00000212287; ENSMUSE00000376310 (ENSEMBL v90)) was deleted using the SpCas9 endonuclease to induce a frameshift mutation. b, Evolutionary history of Efcab3-like in vertebrates including genome structure and surrounding genes. The regions identified in C57BL/6J and C57BL/6NJ (117 and 141, respectively; 145 combined) intersect known GRCm38 assembly issues including gaps, unplaced scaffolds, or centromeric regions (107/145, 73.8%). One of the challenges of gene finders is to distinguish coding genes from pseudogenes and expressed non-coding genes that contain partial open reading frames. 2c). Our de novo assembly is concordant with previously published data for CAST/EiJ48. Similar to Headspace near Portsmouth, ENG. WebHeadspace is a hybrid strain with a 60:40 sativa/indica ratio. Bioinformatics 22, 14371439 (2006). It will take 58 days to mature indoors. PMC The windows were then ordered by density of SNP (lowest, 1 hSNP per 10 kb window, to highest). 2016 May 16;82(11):3297-3309. doi: 10.1128/AEM.00073-16. 17, 167 (2016). 73, 196200 (2012). This resulted in the quantification of 22 unique brain structures, including: (1) total brain area; (2) primary and secondary motor cortices; (3) pons; (4) cerebellar area, internal granular layer of the cerebellum and medial cerebellar nucleus; (5) lateral ventricle; (6) corpus callosum; (7) thalamus; (8) caudate putamen; (9) hippocampus and its associated features; (10) fimbria of the hippocampus; (11) anterior commissure; (12) stria medullaris; (13) fornix; (14) optic chiasm; (15) hypothalamus; (16) pontine nuclei; (17) substantia nigra; (18) fibers of the pons; (19) cingulate cortex; (20) dorsal subiculum; (21) inferior colliculus; and (22) superior colliculus. Approximately 0.52% of total genome length per strain was unplaced and is composed of unknown gap bases (1849%) and repeat sequences (6179%) (Supplementary Table 2), with between 89 and 410 predicted genes per strain (Supplementary Table 3). You are using a browser version with limited support for CSS. 15). Indoors, you can expect to harvest 1-2 Oz/Ft (~ 400 g/m) of weed. 5). Briefly, for each library, 500 ng of high molecular weight genomic DNA (>50 kb mean fragment size) was reconstituted into chromatin in vitro and fixed with formaldehyde. Cactus: Algorithms for genome multiple sequence alignment. 2009 Feb;20(1):15-26. doi: 10.1007/s10532-008-9195-1. However, reliance on mapping next-generation sequencing reads to C57BL/6J has meant that the true extent of strain-specific variation is unknown. WebCannabis sativa (cannabis or marijuana) contains over 100 different terpenes and terpenoids, including mono, sesqui, di and tri, as well as other miscellaneous compounds of terpenoid orgin. Khan MI, Yoo K, Kim S, Cheema SA, Bashir S, Park J. J Microbiol Biotechnol. We used the presence of dense clusters of hSNPs on the C57BL/6J reference genome as a marker for extreme polymorphism and examined the de novo assembly to explore the underlying genomic architecture. CBD is known for its potential therapeutic properties and may help with conditions such as pain, anxiety, inflammation, and seizures. The Schlafen (chromosome 11: 82.983.3 Mb) family of genes are reportedly involved in immune responses, cell differentiation, proliferation and growth, cancer invasion, and chemotherapy resistance. Examining the hSNPs in C57BL/6J and C57BL/6NJ, we find that the vast majority can be explained as occurring in remaining gaps or problematic regions of the reference genome. sharing sensitive information, make sure youre on a federal S.C., C.L., M.T., and B.Y. It can give off an earthy, lemony, pungent, spicy, or dank aroma. This heady hybrid opens the mind and lays out new paths for thinking about yourself and those around you. The .gov means its official. 1, https://doi.org/10.2478/mngs-2013-0001 (2013). Gene sets for each strain were then combined and, using PantherDB76, were classified based on protein class annotations (Fig.